|
| Status |
Public on May 31, 2013 |
| Title |
sera control 2 |
| Sample type |
protein |
| |
|
| Source name |
sera of control mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
tissue: sera
|
| Extracted molecule |
protein |
| Extraction protocol |
1.Collect blood in eppendorf tubes. 2.Leave them at r.t. for 20 min. 3. Spin the tubes at 5,000 rpm x 1 min. 4. Transfer all liquid part into 15 ml conical tubes. 5. Spin the tubes at 3,000 rpm x 10 min. 6. Transfer the serum into one tube (15 or 50 ml tube). Be careful not to contaminate erythrocytes.
|
| Label |
biotin
|
| Label protocol |
protocol see manufacturer's website http://www.raybiotech.com/antibody-array.html
|
| |
|
| Hybridization protocol |
protocol see manufacturer's website http://www.raybiotech.com/antibody-array.html
|
| Scan protocol |
Expose to x-ray film and detect signal using film developer or detect signal directly from membrane using chemiluminescene imaging system.
|
| Data processing |
By comparing the signal intensities, relative expression levels of cytokines can be made. The intensities of signals can be quantified by densitometry. Positive control can be used to normalize the results from different membranes being compared
|
| |
|
| Submission date |
May 30, 2013 |
| Last update date |
May 31, 2013 |
| Contact name |
Qian-qian Yin |
| E-mail(s) |
qqyin@sibs.ac.cn
|
| Phone |
2164154900
|
| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
No280, Chongqing South Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platform ID |
GPL17219 |
| Series (1) |
| GSE47499 |
Analysis of serum cytokine levels of EAE mice |
|
