|
| Status |
Public on Sep 05, 2013 |
| Title |
6hr IFNabR-/- Medium Control Sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM_6hr IFNabR-/- Medium Control
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
genotype/variation: IFNabR-/-
cell type: bone marrow derived macrophages (BMDM)
infected with: none (uninfected) for 6hr
treated with: Media (un-treated control)
|
| Treatment protocol |
cells were infected with Mtb H37Rv or not, with or without IFNgamma (5ng/ml) treatment, for indicated times and harvested into RLT buffer
|
| Growth protocol |
bone marrow cells were cultured for 6 days in L929-cell conditioned media and rested for 1 further day in normal media prior to infection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy minikit
|
| Label |
biotin
|
| Label protocol |
Biotinylated, amplified cRNA were prepared using the Illumina CustomPrep RNA amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
6203221061_B
sample re-run
|
| Data processing |
The data were normalised using 75th percentile normalisation in GeneSpring 11.5
Background subtracted, normalised and log2 transformed for normalised values and non-normalised values have only had background subtraction
|
| |
|
| Submission date |
Jun 05, 2013 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Fin McNab |
| Organization name |
MRC NIMR
|
| Street address |
The Ridgeway, Mill Hill
|
| City |
London |
| ZIP/Postal code |
NW7 1AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE47673 |
TPL-2;ERK1/2 signaling promotes host resistance against intracellular bacterial infection by negative regulation of type I interferon production [Set 2] |
| GSE47674 |
TPL-2;ERK1/2 signaling promotes host resistance against intracellular bacterial infection by negative regulation of type I interferon production |
|
