|
| Status |
Public on Jul 16, 2013 |
| Title |
cell line L-428 |
| Sample type |
RNA |
| |
|
| Source name |
Hodgkin’s lymphoma cell line L-428
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: L428
|
| Growth protocol |
Established Hodgkin's lymphoma cells were grown under standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, biotinylated cDNA was hybridized on Human Exon 1.0ST Microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from established Hodgkin's lymphoma cell line after treatment with vorinostat
|
| Data processing |
The data were analyzed using the RMA algorithm with Affymetrix Expression Console
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
|
| |
|
| Submission date |
Jun 06, 2013 |
| Last update date |
Jul 16, 2013 |
| Contact name |
Martin Sebastian Staege |
| E-mail(s) |
martin.staege@medizin.uni-halle.de
|
| Phone |
+49-345-5577280
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Universitätsklinik u. Poliklinik für Kinder- u. Jugendmedizin
|
| Lab |
Children's Cancer Reserach Center
|
| Street address |
Ernst-Grube-Str. 40
|
| City |
Halle (Saale) |
| State/province |
Sachsen-Anhalt |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE47686 |
Gene expression profiles of Hodgkin’s lymphoma cells |
|
