|
| Status |
Public on Sep 23, 2013 |
| Title |
Tibialis anterior muscle from Neb∆SH3 mouse_rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Tibialis anterior muscle from Neb∆SH3
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed Sv129/Black Swiss
genotype/variation: nebulin SH3 domain deleted (Neb∆SH3)
gender: male
age: 8 weeks
tissue: Tibialis anterior
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent (Invitrogen), following the manufacturer's instructions. Quality control was performed with Agilent Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
Neb∆SH3_2
|
| Data processing |
The data were normalized using the LumiExpresso function of the lumi Bioconductor package in R (R_v.2.15.2, lumi_2.10.0).
|
| |
|
| Submission date |
Jun 10, 2013 |
| Last update date |
Sep 23, 2013 |
| Contact name |
Paolo Kunderfranco |
| E-mail(s) |
Paolo.Kunderfranco@humanitasresearch.it
|
| Organization name |
Humanitas Research Hospital
|
| Department |
Research
|
| Lab |
Bioinformatic Unit
|
| Street address |
Via Rita Levi Montalcini 4
|
| City |
Pieve Emanuele |
| State/province |
Milano |
| ZIP/Postal code |
20090 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE47801 |
Genome-wide gene expression analysis on tibialis anterior muscle from nebulin SH3 domain deleted (Neb∆SH3) mice |
|
