|
| Status |
Public on Jun 10, 2015 |
| Title |
SPF Treg |
| Sample type |
RNA |
| |
|
| Source name |
SPF Treg
|
| Organism |
Mus musculus |
| Characteristics |
strain background: IQI
genotype/variation: specific-pathogen-free
cell type: colonic lamina propria cells
cell subtype: Non-Treg cells; CD3+CD4+FR4+CD25+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL LS or QIAGEN RNeasy micro with Dnase
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Ovation RNA amplification system V2 from at least 20 ng total RNA/
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Description |
RSM14314.CEL
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 11, 2013 |
| Last update date |
Jun 10, 2015 |
| Contact name |
yukihiro furusawa |
| E-mail(s) |
furusawa@pu-toyama.ac.jp
|
| Organization name |
Toyama Prefectural University
|
| Street address |
Kurokawa
|
| City |
Toyama |
| ZIP/Postal code |
939-0398 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE47818 |
Expression data from CD4+ T cells of germ free mice, SPF IQI mice and SPF C57BL/6 mice depletd with Uhrf1 |
|
