|
| Status |
Public on Jun 13, 2013 |
| Title |
Sham, LV, rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Heart tissue from Sham operated dog
|
| Organism |
Canis lupus |
| Characteristics |
tissue: Heart, left ventricle
group: sham operated
|
| Treatment protocol |
Dogs were assigned to right sided uninephrectomy operation or sham operation, heart tissue was harvested at sacrifice 12 weeks after surgery.
|
| Growth protocol |
Normal dogs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix IVT Express Labeling with 100 ng total RNA as input.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol, 16 h hyb @45 degrees C; wash protocol FS450_0001
|
| Scan protocol |
Standard Affymetrix scan protocol
|
| Description |
Gene expression data from left ventricle of sham operated dog, 12 weeks after surgery
|
| Data processing |
The data were analyzed using the Mayo Clinic developed microarray analysis pipleine, MAPP, using fastlo normalization method. MAPP calculates individual mRNA expression profile and eliminates background noise present in raw data as well.
|
| |
|
| Submission date |
Jun 12, 2013 |
| Last update date |
Jun 13, 2013 |
| Contact name |
Asha Nair |
| E-mail(s) |
nair.asha@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Street address |
200 1ST ST SW
|
| City |
Rochester |
| ZIP/Postal code |
55901 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE47876 |
Expression data from uninephrectomized dogs compared to sham operated dogs |
|
