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Sample GSM1163918 Query DataSets for GSM1163918
Status Public on Apr 30, 2014
Title 0.1% DMSO at 72hr, vehicle control, rep1 (human)
Sample type RNA
 
Source name Primary hypatocytes
Organism Homo sapiens
Characteristics tissue: liver
treatment: 0.1% DMSO at 72hr
Treatment protocol After a 24h attachment period, the medium was discarded and replaced by incubation medium, consisting of Dulbecco's Modified Eagle's medium, supplemented with 50 mg/l gentamicin, 4 mg/l insulin, and 10-5 M hydrocortisone and containing 0 [vehicle control, 0.1% (v/v) dimethyl sulfoxide], 30, or 100 µM EMD.
Growth protocol Hepatocytes were plated in 60-mm dishes at a density of 3.5 X 10^6 cell/dish or in 6-well BD BioCoat plated (BD Biosciences, San Jose, CA) at a density of 1.5 X 10^6 cells/well in attachment medium and cultured under a CO2/air (5%/95%) humidified atmosphere at 37oC.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description H9
Gene expression data from human primary hypatocytes.
Data processing The data were analyzed with the Affymetrix Console using RMA summarization.
 
Submission date Jun 14, 2013
Last update date Apr 30, 2014
Contact name John Okyere
E-mail(s) john.okyere@crossgen.co.uk
Organization name CrossGen Limited
Street address BioCity Nottingham
City Nottingham
ZIP/Postal code NG1 1GF
Country United Kingdom
 
Platform ID GPL570
Series (2)
GSE47970 Cross-species gene expression analysis of species-specific differences in preclinical assessment of pharmaceutical compounds (human)
GSE47972 Cross-species gene expression analysis of species-specific differences in preclinical assessment of pharmaceutical compounds

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.83648
AFFX-BioB-M_at 7.50536
AFFX-BioB-3_at 7.12368
AFFX-BioC-5_at 8.67966
AFFX-BioC-3_at 8.6155
AFFX-BioDn-5_at 9.84524
AFFX-BioDn-3_at 10.9453
AFFX-CreX-5_at 12.3499
AFFX-CreX-3_at 12.6062
AFFX-DapX-5_at 8.21086
AFFX-DapX-M_at 9.22227
AFFX-DapX-3_at 9.88264
AFFX-LysX-5_at 5.11023
AFFX-LysX-M_at 5.99642
AFFX-LysX-3_at 7.37803
AFFX-PheX-5_at 6.44524
AFFX-PheX-M_at 6.67837
AFFX-PheX-3_at 6.93687
AFFX-ThrX-5_at 7.03606
AFFX-ThrX-M_at 7.40829

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM1163918_09H.CEL.gz 6.8 Mb (ftp)(http) CEL
GSM1163918_09H.rma.chp.gz 371.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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