|
| Status |
Public on Jun 18, 2013 |
| Title |
IRfl/fl Cre- 4 |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mammary alveolar cell (MEC)
|
| Treatment protocol |
Three mice of each strain were sacrificed on day 13.5 of pregnancy.
Mammary epithelial cells were isolated with a collagenase treatment from day 13.5 mice.
|
| Growth protocol |
Female mice of the strains IRfl/fl Cre- and IRfl/fl Cre+ were bred with CD1 males.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated according to the method: Rudolph MC, et al. J Mammary Gland Biol Neoplasia 8: 287-307, 2003.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) and scanned at the UCLA Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; core-exon analysis
Cre- .4
|
| Data processing |
Raw data were processed with the Exon Array Computational Tool (ExACT) (Affymetrix) for background correction and normalization. Data analysis and statistical evaluations were performed as described on the Series record.
|
| |
|
| Submission date |
Jun 17, 2013 |
| Last update date |
Jun 18, 2013 |
| Contact name |
Margaret C Neville |
| E-mail(s) |
peggy.neville@ucdenver.edu
|
| Phone |
970 468 0668
|
| Organization name |
UCDenver
|
| Department |
OB/GYN
|
| Street address |
712 Eveningstar Rd
|
| City |
Silverthorne |
| State/province |
CO |
| ZIP/Postal code |
80498 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE48009 |
Gene expression in IRfl/fl Cre- and IRfl/fl Cre+ mice |
|
