|
| Status |
Public on Oct 31, 2006 |
| Title |
Pregnant females exposed to ethanol for 5 days, AF03 (Alcohol Fed) corresponds to replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Alcohol Fed 03
|
| Organism |
Mus musculus |
| Characteristics |
Total RNA extracted from the cerebral cortex of timed pregnant C57B16 mice exposed to alcohol
|
| Treatment protocol |
Timed pregnant C57Bl6 mice exposed to about 180-200mg/dl ethanol for 4 days corresponding to gestation day 15 to 18
|
| Growth protocol |
Time pregnant C57Bl6 mice were fed adlibitum with food and water prior to ethanol exposure (corresponding to gestational day 14)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted with Ambion's miRNA isolation kit
|
| Label |
Cy5
|
| Label protocol |
1ug of total RNA reverse-transcribed and labeled according Genisphere's 3DNA Array 900 Cy3/Cy5 kit's protocol
|
| |
|
| Channel 2 |
| Source name |
Weight matched control
|
| Organism |
Mus musculus |
| Characteristics |
Total RNA extracted from the cerebral cortex of 7 animals were combined and to be used as a normalization control
|
| Treatment protocol |
Pregnant animals were fed adlibitum with food and water (control)
|
| Growth protocol |
Time pregnant C57Bl6 mice were fed adlibitum with food and water prior to the experiment and during the experiment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted with Ambion's miRNA isolation kit
|
| Label |
Cy3
|
| Label protocol |
A total of 1ug of total RNA was reverse-transcribed with Superscript II (Invitrogen) using primers for Cy3 and Cy5 according to the 3DNA Array 900 Cy3/Cy5 protocol (Genisphere).
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed according to Genisphere's 3DNA Array 900 kit
|
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 6.0.1.26 analysis software
|
| Description |
Timed pregnant C57Bl6 mice were exposed to ethanol during gestational day 15 to day 18 and ethanol exposed fetuses were delivered by cessarian section on gestational day 19. Alcohol exposed fetuses were compared to control timed pregnant mice and to pair fed controls (animals fed with maltose dextrose)
|
| Data processing |
Background correction and removal of flagged values were conducted prior to any further analysis
|
| |
|
| Submission date |
Jun 28, 2006 |
| Last update date |
Dec 03, 2021 |
| Contact name |
Rajesh Miranda |
| E-mail(s) |
rmiranda@tamu.edu
|
| Organization name |
Texas A&M University
|
| Street address |
8447 Riverside Parkway
|
| City |
Bryan |
| ZIP/Postal code |
77807 |
| Country |
USA |
| |
|
| Platform ID |
GPL3919 |
| Series (1) |
| GSE5186 |
Gene expression changes in fetuses exposed to ethanol in utero |
|
