|
| Status |
Public on Jun 20, 2013 |
| Title |
PANC-1 cells 24 hours post GLV-1h153 infection rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PANC-1 cells incubated in media
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pancreas/duct
morphology: epithlial
disease: epithelioid carcinoma
cell line: PANC-1
time: 24 h
infection: GLV-1h153
|
| Treatment protocol |
Cells were infected with GLV-1h153 at a multiplicity of infection of 5.0 and incubated at for zero, 6 and 24 hours.
|
| Growth protocol |
PANC-1 cells (American type culture collection) were plated at 5 × 105 cells per well in 6-well plates and grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 1% antibiotic-antimycotic solution (Mediatech, Inc., Herndon, VA) and 10% fetal bovine serum (FBS) (Mediatech, Inc.) at 37oC under 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Qiagen Inc., Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2002, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133A Array.
|
| Scan protocol |
HG-U133A cDNA microarray chip images were scanned and processed to CEL files using the standard GCOS analysis suite (Affymetrix Inc).
|
| Description |
PANC-1 cells 24 hours after infection with GLV-1h153
|
| Data processing |
CEL files were then normalized and processed to signal intensities using the gcRMA algorithm from the Bioconductor library for the R statistical programming system.
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| |
|
| Submission date |
Jun 19, 2013 |
| Last update date |
Jun 20, 2013 |
| Contact name |
Dana Hadadd |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Department |
Surgery
|
| Lab |
Yuman Fong
|
| Street address |
410 E 69th st 4th floor
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10019 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE48121 |
Expression data from human pancreatic cells PANC-1 infected with oncolytic vaccinia virus GLV-1h153 |
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