|
| Status |
Public on Jun 23, 2014 |
| Title |
P53_cKO_2 |
| Sample type |
RNA |
| |
|
| Source name |
P53 knock-out
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: P53 knock-out; Cdh1+, P53-
age: 2 months
gender: female
tissue: uterus
|
| Treatment protocol |
Female mice were genotyped and necropsied at 2 month of age. Uterine tissues were homogenized with Trizol reagent at collection and stored at-80C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
P74
|
| Data processing |
The data were processed in R (2.14.1) using limma (3.10.2) using the neqc function, which does a normexp background correction using negative control probes, quantile normalization using negative and positive control probes and log2 transformation.
|
| |
|
| Submission date |
Jun 19, 2013 |
| Last update date |
Jun 23, 2014 |
| Contact name |
Kanako Hayashi |
| E-mail(s) |
k.hayashi@wsu.edu
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
BLS345, 1770 NE Stadium Way
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE48131 |
Ablation of Cdh1 and Trp53 in the uterus reveals novel mechanisms controlling tumor microenvironment in endometrial cancer. |
|
