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Sample GSM116946 Query DataSets for GSM116946
Status Public on Jun 29, 2007
Title R. palustris hydrogen-producing mutant (CGA570) Replicate #1
Sample type RNA
 
Source name Photosynthetic bacterium Rhodopseudomonas palustris
Organism Rhodopseudomonas palustris
Characteristics R. palustris hydrogen-producing mutant (CGA570) Replicate #1
Growth protocol Photosynthetically grown with acetate and ammonium.
Extracted molecule total RNA
Extraction protocol Rhodopseudomonas palustris cells were disrupted by treatment with by bead beating and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
Label Biotinylated GeneChip DNA labeling reagent
Label protocol As recommended by the manufacturer (Affymetrix)
 
Hybridization protocol As recommended by the manufacturer (Affymetrix)
Scan protocol As recommended by the manufacturer (Affymetrix)
Description cDNA synthesis, fragmentation, labeling, hybridization, and processing of GeneChips were carried out as recommended by the manufacture (Affymetrix).
Data processing The Affymetrix Microarray Suite was used for initial data acquisition and processing.
 
Submission date Jun 29, 2006
Last update date Feb 05, 2007
Contact name Yasuhiro Oda
E-mail(s) yasuhiro@uw.edu
Organization name University of Washington
Department Department of Microbiology
Lab Harwood
Street address Box 357242, 1705 N.E. Pacific Street, HSB K-354
City Seattle
State/province WA
ZIP/Postal code 98195-7242
Country USA
 
Platform ID GPL3954
Series (1)
GSE5194 Redirection of metabolism for biological hydrogen production

Data table header descriptions
ID_REF
VALUE signal intensity
ABS_CALL detection

Data table
ID_REF VALUE ABS_CALL
RPA0001_at 1351.2 P
RPA0002_at 2540.5 P
RPA0003_at 426.7 A
RPA0004_at 936.8 P
RPA0005_at 164.3 A
RPA0006_at 136.7 A
RPA0007_at 323.1 A
RPA0008_at 762.1 A
RPA0009_at 662 A
RPA0010_at 906.9 P
RPA0011_at 242.1 A
RPA0012_at 832 A
RPA0013_at 552.1 A
RPA0014_at 639 P
RPA0015_at 865.2 P
RPA0016_at 3197.1 P
RPA0017_at 2166.9 P
RPA0018_at 2610.5 P
RPA0019_at 1833.7 P
RPA0020_at 336 P

Total number of rows: 4816

Table truncated, full table size 88 Kbytes.




Supplementary file Size Download File type/resource
GSM116946.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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