|
Status |
Public on Jun 29, 2007 |
Title |
R. palustris hydrogen-producing mutant (CGA570) Replicate #1 |
Sample type |
RNA |
|
|
Source name |
Photosynthetic bacterium Rhodopseudomonas palustris
|
Organism |
Rhodopseudomonas palustris |
Characteristics |
R. palustris hydrogen-producing mutant (CGA570) Replicate #1
|
Growth protocol |
Photosynthetically grown with acetate and ammonium.
|
Extracted molecule |
total RNA |
Extraction protocol |
Rhodopseudomonas palustris cells were disrupted by treatment with by bead beating and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
|
Label |
Biotinylated GeneChip DNA labeling reagent
|
Label protocol |
As recommended by the manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
As recommended by the manufacturer (Affymetrix)
|
Scan protocol |
As recommended by the manufacturer (Affymetrix)
|
Description |
cDNA synthesis, fragmentation, labeling, hybridization, and processing of GeneChips were carried out as recommended by the manufacture (Affymetrix).
|
Data processing |
The Affymetrix Microarray Suite was used for initial data acquisition and processing.
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|
|
Submission date |
Jun 29, 2006 |
Last update date |
Feb 05, 2007 |
Contact name |
Yasuhiro Oda |
E-mail(s) |
yasuhiro@uw.edu
|
Organization name |
University of Washington
|
Department |
Department of Microbiology
|
Lab |
Harwood
|
Street address |
Box 357242, 1705 N.E. Pacific Street, HSB K-354
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98195-7242 |
Country |
USA |
|
|
Platform ID |
GPL3954 |
Series (1) |
GSE5194 |
Redirection of metabolism for biological hydrogen production |
|