|
| Status |
Public on Jun 29, 2007 |
| Title |
R. palustris hydrogen-producing mutant (CGA570) Replicate #2 |
| Sample type |
RNA |
| |
|
| Source name |
Photosynthetic bacterium Rhodopseudomonas palustris
|
| Organism |
Rhodopseudomonas palustris |
| Characteristics |
R. palustris hydrogen-producin mutant (CGA570) Replicate #2
|
| Growth protocol |
Photosynthetically grown with acetate and ammonium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rhodopseudomonas palustris cells were disrupted by treatment with by bead beating and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
|
| Label |
Biotinylated GeneChip reagent
|
| Label protocol |
As recommended by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As recommended by the manufacturer (Affymetrix)
|
| Scan protocol |
As recommended by the manufacturer (Affymetrix)
|
| Description |
cDNA synthesis, fragmentation, labeling, hybridization, and processing of GeneChips were carried out as recommended by the manufacture (Affymetrix).
|
| Data processing |
The Affymetrix Microarray Suite was used for initial data acquisition and processing.
|
| |
|
| Submission date |
Jun 29, 2006 |
| Last update date |
Feb 05, 2007 |
| Contact name |
Yasuhiro Oda |
| E-mail(s) |
yasuhiro@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Microbiology
|
| Lab |
Harwood
|
| Street address |
Box 357242, 1705 N.E. Pacific Street, HSB K-354
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-7242 |
| Country |
USA |
| |
|
| Platform ID |
GPL3954 |
| Series (1) |
| GSE5194 |
Redirection of metabolism for biological hydrogen production |
|
