|
| Status |
Public on Jun 25, 2013 |
| Title |
Fludara CLL1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary CLL cells (treated with 10 μM fludarabine for 4h)
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 10 μM fludarabine for 4h
cell type: CLL B-cells
|
| Treatment protocol |
5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
|
| Growth protocol |
Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 24, 2013 |
| Last update date |
Jun 25, 2013 |
| Contact name |
Chris Pepper |
| E-mail(s) |
peppercj@cf.ac.uk
|
| Phone |
+44 2920687057
|
| Organization name |
Cardiff University
|
| Street address |
Heath Park
|
| City |
Cardiff |
| ZIP/Postal code |
CF14 4XN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE48258 |
Changes in gene expression induced by CDK9 inhibition alone and in combination with fludarabine |
|
