|
| Status |
Public on Jun 23, 2020 |
| Title |
MC2494 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
acute myeloid leukemia
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: MC2494
cell line: U937
|
| Treatment protocol |
U937 clls were treated with the compounds for 24h
|
| Growth protocol |
U937 cells were cultured in RPMI medium supplemented with 2 mM glutamine, 100 IU/ml penicillin, 100 µg/ml streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using TRIZOL (Invitrogen) reagent following manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
3000ng of total RNA were converted in cDNA and then in cRNA following manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
chip were scanned with Illumina BeadArray Reader, BeadScan software version 3.5.49
|
| Description |
replicate 2
SAMPLE 10
|
| Data processing |
The data were normalised using quantile normalisation with limma package in R
|
| |
|
| Submission date |
Jun 25, 2013 |
| Last update date |
Jun 23, 2020 |
| Contact name |
Annamaria Carissimo |
| E-mail(s) |
carissimo@tigem.it
|
| Organization name |
Tigem
|
| Street address |
via Castellino
|
| City |
Napoli |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE48295 |
Genes differentially expressed in human AML cells after treatment with a novel SirTi, MC2494. |
|
