|
| Status |
Public on Jun 27, 2013 |
| Title |
Human granulocytes b |
| Sample type |
SRA |
| |
|
| Source name |
granulocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Promyelocytes, myelocytes and granulocytes were purified using fluorescence activated cell sorting. RNA was extracted using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
C3.Human.transcripts.gtf.bz2 available on the Series record
|
| Data processing |
Illumina Pipeline 1.5 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, then mapped to mm8 whole genome using bowtie v0.12.7
Alternative transcription start sites and gene expression were determined using Cufflinks Version 0.92.
Genome_build: mm9 & hg19
Supplementary_files_format_and_content: GTF file of transcripts
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
william ritchie |
| Organization name |
centenary institute
|
| Street address |
Building 93
|
| City |
Camperdown |
| State/province |
NSW |
| ZIP/Postal code |
2050 |
| Country |
Australia |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE48305 |
Orchestrated intron retention regulates normal granulocyte differentiation [RNA-Seq] |
| GSE48307 |
Orchestrated intron retention regulates normal granulocyte differentiation |
|
| Relations |
| BioSample |
SAMN02213910 |
| SRA |
SRX315144 |
