|
| Status |
Public on Jun 27, 2013 |
| Title |
Y31301 (T2) |
| Sample type |
RNA |
| |
|
| Source name |
Vehicle, T2
|
| Organism |
Macaca fascicularis |
| Characteristics |
tissue: Blood
treatment: Vehicle
time (h): 2
subject: Y31301
|
| Treatment protocol |
Collection blood on PAXgene tube
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the PAXgene Blood RNA (Qiagen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard NuGEN Ovation® Whole Blood Solution with Biotin kit protocol from 50 ng total RNA .
|
| |
|
| Hybridization protocol |
Following fragmentation,4.4 ug of cDNA were hybridized for 18hr at 45°C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the Gene chip Scanner 3000 affymetrix
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Jun 27, 2013 |
| Contact name |
Marc Derive |
| E-mail(s) |
marc.derive@inserm.fr
|
| Organization name |
Inserm U1116
|
| Street address |
9, av de la Forêt de Haye
|
| City |
Vandoeuvre-lès-Nancy |
| ZIP/Postal code |
54500 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE48311 |
Time-dependent changes in gene expression after endotoxinic challenge followed by LR12-scrambled or LR12 treatment. |
|
