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Sample GSM1174670 Query DataSets for GSM1174670
Status Public on Jun 27, 2013
Title Y31304 (T8)
Sample type RNA
 
Source name Vehicle, T8
Organism Macaca fascicularis
Characteristics tissue: Blood
treatment: Vehicle
time (h): 8
subject: Y31304
Treatment protocol Collection blood on PAXgene tube
Extracted molecule total RNA
Extraction protocol Total RNA extraction was performed using the PAXgene Blood RNA (Qiagen) according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cDNA were prepared according to the standard NuGEN Ovation® Whole Blood Solution with Biotin kit protocol from 50 ng total RNA .
 
Hybridization protocol Following fragmentation,4.4 ug of cDNA were hybridized for 18hr at 45°C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
Scan protocol GeneChips were scanned using the Gene chip Scanner 3000 affymetrix
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Jun 26, 2013
Last update date Jun 27, 2013
Contact name Marc Derive
E-mail(s) marc.derive@inserm.fr
Organization name Inserm U1116
Street address 9, av de la Forêt de Haye
City Vandoeuvre-lès-Nancy
ZIP/Postal code 54500
Country France
 
Platform ID GPL570
Series (1)
GSE48311 Time-dependent changes in gene expression after endotoxinic challenge followed by LR12-scrambled or LR12 treatment.

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 806.919 P 4.42873e-05
AFFX-BioB-M_at 1459.68 P 4.42873e-05
AFFX-BioB-3_at 1270.54 P 6.02111e-05
AFFX-BioC-5_at 2893.63 P 4.42873e-05
AFFX-BioC-3_at 2780.83 P 4.42873e-05
AFFX-BioDn-5_at 5503.13 P 4.42873e-05
AFFX-BioDn-3_at 8279.49 P 4.42873e-05
AFFX-CreX-5_at 17948.8 P 4.42873e-05
AFFX-CreX-3_at 16668.6 P 4.42873e-05
AFFX-DapX-5_at 1618.52 P 4.42873e-05
AFFX-DapX-M_at 3388.25 P 6.02111e-05
AFFX-DapX-3_at 5408.05 P 4.42873e-05
AFFX-LysX-5_at 561.039 P 4.42873e-05
AFFX-LysX-M_at 1143.39 P 4.42873e-05
AFFX-LysX-3_at 1369.19 P 4.42873e-05
AFFX-PheX-5_at 323.836 P 4.42873e-05
AFFX-PheX-M_at 533.178 P 4.42873e-05
AFFX-PheX-3_at 1042.4 P 4.42873e-05
AFFX-ThrX-5_at 502.297 P 5.16732e-05
AFFX-ThrX-M_at 616.159 P 4.42873e-05

Total number of rows: 54675

Table truncated, full table size 1623 Kbytes.




Supplementary file Size Download File type/resource
GSM1174670_NUID-0000-39070-0064.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM1174670_NUID-0000-39070-0064.mas5.CHP.gz 501.5 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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