Human neural stem cells (CTX066), provided by Dr. Clive Svendsen (Waisman Center, University of Wisconsin at Madison), were generated from human embryonic cortical tissue (8 weeks post conception). The neurospheres were passaged every 14 days by sectioning into 150 µM section, which then were reseeded into fresh growth medium containing 70% DMEM, 30% Hams F12, 1% penicillin/streptomycin/amphotericin B supplemented with 20 µg/ml EGF, 10 ng/ml LIF and 1% v/v N2 at a density equivalent to 200,000 cells per ml. Half the growth medium was replenished every four days. The cells were treated with tert-butylhydroquinone (20µM) for 24 hours and harvested on Jan. 16 2002 Keywords = human neural stem cell Keywords = tert-butylhydroquinone Keywords = antioxidant responsive element
