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Sample GSM1179388 Query DataSets for GSM1179388
Status Public on Aug 30, 2013
Title 1000nM_Topotecan
Sample type RNA
Source name cultured cortical neurons
Organism Mus musculus
Characteristics treatment: 1000nM_Topotecan
cell type: cultured cortical neurons
strain: C57BL6
Treatment protocol After 7 days in vitro, mouse cortical neurons were treated with drug or vehicle. After 24 or 72 hrs drug treatement, total RNA was prepared.
Growth protocol Mouse cortical neurons were cultured from E13-15 mouse embryos for 7 days in vitro.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse cortical neurons using Trizol reagent.
Label biotin
Label protocol RNA was labeled using the Affymetrix/Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit.
Hybridization protocol Arrays for experiments (1) and (3) were washed and stained using the Affymetrix Gene Fluidics station 450. Hybridization was performed at 48˚C for 17 hours. Arrays for other experiments were run using the Affymetrix GeneTitan system. Steps subsequent to hybridization were performed with the Beckman Coulter FXP Target Express Robot. Hybridization was performed at 45˚C for 16 hours with the Affymetrix GeneTitan instrument, controlled using AGCC software.
Scan protocol GeneTitan arrays were scanned using the Affymetrix GeneTitan instrument, controlled by AGCC software. Individual arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with autoloader.
Description MoGene-1_1-st-v1
Gene Expression data for Topotecan treated mouse cultured cortical neurons
Data processing Arrays were normalized and expression values determined using Affymetrix Expression Console. Linear RMA normalization was used for all experiments.
Submission date Jul 02, 2013
Last update date Aug 30, 2013
Contact name Chandri Yandava
Organization name University of North Carolina at Chapel Hill
Department Carolina Institute for Developmental Disabilities
Street address 101 Renee Lynne Court
City Carrboro
State/province NC
ZIP/Postal code 27510
Country USA
Platform ID GPL11533
Series (2)
GSE43899 Co-ordinate inhibiton of autism candidate genes by topoisomerase inhibitors [array]
GSE43900 Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors

Data table header descriptions
VALUE Linear RMA signal

Data table
10344614 103.3714
10344616 5.675869
10344620 10.18419
10344622 26.31051
10344624 673.8636
10344633 588.887
10344637 1106.667
10344653 14.54923
10344658 446.548
10344674 12.18324
10344679 34.71442
10344707 808.1032
10344713 745.7086
10344715 25.88288
10344717 10.34183
10344719 21.02784
10344721 6.216936
10344723 72.81554
10344725 367.8153
10344741 1075.266

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.

Supplementary file Size Download File type/resource
GSM1179388_1000nM_Topotecan.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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