|
| Status |
Public on Jul 10, 2013 |
| Title |
IUGR islets, e19, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
IUGR surgery rat islets, pooled from 1 litter
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
treatment: IUGR surgery e19 rat islets, pooled from 1 litter
tissue: pancreatic islet
age: e19
|
| Treatment protocol |
4 dams were underwent IUGR surgery, 4 dams underwent sham surgery on e18.
|
| Growth protocol |
8 timed pregnant dams purchased from Charles River Laboratories
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from pooled fetal islets at e19 (n=4 different litters per treatment) using RNAzol B.
|
| Label |
biotin
|
| Label protocol |
GeneChip WT terminal labeling kit
|
| |
|
| Hybridization protocol |
standard protocol for Rat Gene 1.0 ST
|
| Scan protocol |
standard protocol for Rat Gene 1.0 ST
|
| Description |
IUGR surgery e19 rat islets, pooled from 1 litter
|
| Data processing |
RMA, then samr v2.0
|
| |
|
| Submission date |
Jul 09, 2013 |
| Last update date |
Jul 10, 2013 |
| Contact name |
Lane Johanna Jaeckle Santos |
| E-mail(s) |
ljaeck@mail.med.upenn.edu
|
| Phone |
215-746-5138
|
| Organization name |
University of Pennsylvania School of Medicine
|
| Department |
CRRWH
|
| Lab |
Rebecca Simmons'
|
| Street address |
421 Curie Blvd, BRB II/III 1341
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE48655 |
Expression data from growth restricted fetal rat pancreatic islets |
|
