|
| Status |
Public on Dec 21, 2013 |
| Title |
Animal-blastomeres_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Animal Blastomeres
|
| Organism |
Xenopus laevis |
| Characteristics |
developmental stage: 8-cell embryos
tissue: animal blastomeres
|
| Treatment protocol |
Jelly coat was removed by incubation at room temp. in 2% cysteine, pH 8.0 for 4 minutes, and fixed in 98% ethanol, 2% acetic acid for dissections.
|
| Growth protocol |
Embryos were collected after hormone-induced natural matings of 2-pairs of adult Xenopus laevis,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNeasy mini kit (Qiagen) using manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNAs were labeled and fragmented with the Ovation Biotin RNA Amplification and Labeling System (NuGEN Technologies, Inc.) according to standard protocol.
|
| |
|
| Hybridization protocol |
The labeled cDNAs were purified with the Dye Ex kit (Qiagen), and shipped to the NINDS-NIMH Microarray Consortium at The Translational Genomics Research Institute (T-GEN, Tempe, AZ).
|
| Scan protocol |
The chips were washed and scanned as recommended by the Ovation Biotin RNA Amplification and Labeling System User Guide (version 1.0).
|
| Description |
NIH-Moody-A2-Xen
Maternally deposited RNAs present in animal blastomeres
|
| Data processing |
GCOS software was used to determine signal intensities and detection calls for each gene. Please note that the data was processed by a service facility, who no longer exists. Thus, the submitter is not sure about software version or algorithm used for processing.
|
| |
|
| Submission date |
Jul 10, 2013 |
| Last update date |
Dec 22, 2013 |
| Contact name |
Sally A. Moody |
| E-mail(s) |
samoody@gwu.edu
|
| Phone |
2029942878
|
| Organization name |
The George Washington University School of Medicine and Health Sciences
|
| Department |
Anatomy and Regenerative Biology
|
| Lab |
Moody
|
| Street address |
2300 I St. NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE48659 |
Comparison of animal-blastomeres' RNA content to vegetal-most half of vegetal blastomeres' RNA content at the 8-cell stage of Xenopus laevis embryos to identify animal-enriched transcripts |
|
