Human neuroblastoma cells, IMR-32, were grown in Dulbecco's modified eagle's medium (DMEM) supplemented with fetal calf serum (10%), 100 I.U./ml penicillin, and 100 mg/ml streptomycin. Cultures were maintained at 37 C in a humidified 10% CO2 atmosphere. The cells were treated with EtOH (0.01%) or tBHQ (10µM) for 24 hours and harvested for microarray analysis.
