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Status |
Public on Apr 27, 2015 |
Title |
Sample 1 PRA and PRB |
Sample type |
RNA |
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Source name |
Human endometrial stromal cell sample 1 expressing flag-PRA and flag-PRB
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Organism |
Homo sapiens |
Characteristics |
cell type: endometrial stromal cell treatment: PGR kd + PRA and PRB expression
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Treatment protocol |
treatment in 2% charcoal stripped serum sontaining DMEM:F12 medium, 48h siRNA transfection, 24h adenovirus transduction, 6h differentiation cocktail treatment composed of E, P and cAMP
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Growth protocol |
growth in 5%FBS containing DMEM:F12 medium
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was performed using TRIzol® according to the manufacturer's instructions. Total RNA was cleaned up using RNeasy Mini Kit (Qiagen) following manufacturer's instructions
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Label |
biotin
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Label protocol |
The Keck Center Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign prepared amplified, biotin-labeled, fragmented antisense RNA from 100 nanograms of sample total RNA using the Affymetrix 3' Express Kit (Affymetrix, Inc., Santa Clara, CA) according to manufacturer’s instructions.
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Hybridization protocol |
10 micrograms of labeled aRNA were hybridized to a Human Genome HG-U133 A2.0 Array . Wash and stain were performed on Affymetrix Fluidics Station 450 with protocol EukGE_WS2v5 using Affymetrix GeneChip Command Console Fluidics Control software and solutions prepared by Keck Center,Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.
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Scan protocol |
Arrays were scanned on Affymetrix GeneChip Scanner model 3000 7G Plus using GeneChip Command Console Scan Control software to generate DAT and CEL files.
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Description |
Human endometrial stromal cell, decidualization, PGR PRA and PRB expressing, 6h differentiation
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Data processing |
To generate quality control metrics the Keck Center generated CHP files using the MAS5 algorithm with Affymetrix Expression Console version 1_1. The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix Expression Console with default analysis settings.
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Submission date |
Jul 14, 2013 |
Last update date |
Apr 27, 2015 |
Contact name |
Milan K Bagchi |
Organization name |
UIUC
|
Department |
MIP
|
Lab |
Bagchi Lab 534 Burrill Hall
|
Street address |
407 S. Goodwin Ave
|
City |
Urbana |
State/province |
IL |
ZIP/Postal code |
61801 |
Country |
USA |
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Platform ID |
GPL571 |
Series (1) |
GSE48853 |
Expression data of PGR isoforms, PRA and PRB, regulated genes in differentiating human endometrial stromal cells |
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