|
| Status |
Public on Sep 03, 2013 |
| Title |
DP185-21% miRNA |
| Sample type |
RNA |
| |
|
| Source name |
DP185-21%
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: dental pulp cells
gender: male
|
| Growth protocol |
Dental pulp cells were cultured in MSCGM medium (LONZA) under 21% and 3% oxygen concentration atomosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an miRNeasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
After dephosphorylation and denaturation, the total RNA was labeled with cyanine 3-pCp,
|
| |
|
| Hybridization protocol |
and then hybridized to Agilent Microarray Sure Print G3 Human miRNA 8 × 60 K miRBase 16.0 using the miRNA Complete Labeling and Hyb Kit (Agilent Technologies).
|
| Scan protocol |
Following hybridization for 20 h, the slides were washed using the Gene Expression Wash Buffer Kit (Agilent Technologies) and scanned using an Agilent Scanner.
|
| Description |
Gene expression under 21% O2
|
| Data processing |
The images were processed and analyzed with Agilent Feature Extraction Software. The raw data were normalized using quantile normalization and then analyzed in GeneSpring GX software 11.5 (Agilent Technologies).
|
| |
|
| Submission date |
Jul 15, 2013 |
| Last update date |
Sep 03, 2013 |
| Contact name |
Ken-ichi Tezuka |
| E-mail(s) |
tezuka@gifu-u.ac.jp
|
| Phone |
+81-58-230-6476
|
| Organization name |
Gifu University Graduate School of Medicine
|
| Department |
Gifu University Graduate School of Medicine
|
| Street address |
1-1 Yanagido
|
| City |
Gifu City |
| State/province |
Gifu |
| ZIP/Postal code |
501-1194 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15018 |
| Series (1) |
| GSE45872 |
Hypoxia-enhanced Derivation of iPSCs from Human Dental Pulp Cells |
|
