|
| Status |
Public on Jul 18, 2013 |
| Title |
MCF7 control siRNA Estradiol Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Control siRNA Estradiol
cell line: MCF7
|
| Treatment protocol |
MCF7 cells were transfected with control or cyclin D1 siRNA. Cells were maintained for 48hrs to allow for efficient knock-down. Estradiol or vehicle treated cells were maintained for a further 24 hrs and then processed.
|
| Growth protocol |
MCF7 cells grown in phenol red free DMEM supplemented with 5% charcoal stripped media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of RNA was performed as per manufacturer's instructions and purified using Qiagen RNAeasy Mini kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to standard NuGEN protocol from 50ug total RNA.
|
| |
|
| Hybridization protocol |
2.5ug of ST cDNA were hybridized for 18h at 45 degrees on theAffymetrix Gene ChIP Human gene 1.0 ST Array
|
| Scan protocol |
Arrays were inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000 with Command Console Software
|
| Data processing |
Raw intensity files from the Affymetrix Human Gene 1.0 ST GeneChip arrays were processed using Affymetrix Expression Console version 1.1. Gene-level expression measurements were computed using the iterPLIER algorithm on the “core” probesets and exported
|
| |
|
| Submission date |
Jul 17, 2013 |
| Last update date |
Jul 18, 2013 |
| Contact name |
Mathew Casimiro Casimiro |
| Organization name |
Thomas Jefferson University
|
| Department |
233 South 10th St, Rm 1035
|
| Lab |
Philadelphia
|
| Street address |
233 South 10th St, Rm 1035
|
| City |
19107 |
| State/province |
United States |
| ZIP/Postal code |
Mathew Casimiro |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE48989 |
Cyclin D1 determines estrogen dependent signaling in human breast cancer cell line MCF7 |
|
