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Sample GSM119285 Query DataSets for GSM119285
Status Public on Jul 10, 2006
Title Lung from SM22-dnBMPR2 inducible transgenic males activated 1 week
Sample type RNA
Source name lung
Organism Mus musculus
Characteristics A pool of RNA from lung from three male mice, raised at Denver altitude (nearly normoxic), with SM22-rtTA x tetO7-BMPR2delx4+ transgene activated for 1 week. These mice have elevated RV pressures, and dysfunctional pulmonary vascular reactivity.
Extracted molecule total RNA
Extraction protocol Lung tissues were flash-frozen in liquid nitrogen within minutes of death, and RNA extracted using an RNeasy kit (Qiagen)
Label Biotin
Label protocol First and second strand complimentary DNA was synthesized using standard techniques. Biotin-labeled antisense complimentary RNA was produced by an in vitro transcription reaction.
Hybridization protocol Mouse Genome 430 2.0 microarrays (Affymetrix, Foster City, CA) were hybridized with 20 �g cRNA. Target hybridization, washing, staining, and scanning probe arrays were done following an Affymetrix GeneChip Expression Analysis Manual.
Description Lung from SM22-dnBMPR2 inducible transgenic males activated 1 week
Data processing dChip, using PM/MM algorithm
Submission date Jul 06, 2006
Last update date Jul 07, 2006
Contact name James West
Organization name Vanderbilt University
Department Medicine
Lab Pulmonary
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
Platform ID GPL1261
Series (1)
GSE5255 Loss of BMPR2 Signaling in Smooth Muscle in Mouse Lung

Data table header descriptions
VALUE Lung from SM22-dnBMPR2 inducible transgenic males activated 1 week

Data table
1426906_at 425.54
1425451_s_at 164.14
1435330_at 218.94
1450826_a_at 233.04
1456471_x_at 197.14
1454714_x_at 178.55
1419764_at 383.33
1452231_x_at 666.3
1449824_at 114.93
1422412_x_at 396.24
1421791_at 76.83
1419627_s_at 80.04
1417556_at 40.79
1460227_at 221.85
1444176_at 53.07
1422411_s_at 672.58
1434798_at 55.91
1434484_at 187.16
1422953_at 121.72
1420249_s_at 272.03

Total number of rows: 45037

Table truncated, full table size 764 Kbytes.

Supplementary data files not provided

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