A pool of RNA from lung from three male mice, raised at Denver altitude (nearly normoxic), with SM22-rtTA x tetO7-BMPR2delx4+ transgene activated for 1 week. These mice have elevated RV pressures, and dysfunctional pulmonary vascular reactivity.
Extracted molecule
total RNA
Extraction protocol
Lung tissues were flash-frozen in liquid nitrogen within minutes of death, and RNA extracted using an RNeasy kit (Qiagen)
Label
Biotin
Label protocol
First and second strand complimentary DNA was synthesized using standard techniques. Biotin-labeled antisense complimentary RNA was produced by an in vitro transcription reaction.
Hybridization protocol
Mouse Genome 430 2.0 microarrays (Affymetrix, Foster City, CA) were hybridized with 20 �g cRNA. Target hybridization, washing, staining, and scanning probe arrays were done following an Affymetrix GeneChip Expression Analysis Manual.
Description
Lung from SM22-dnBMPR2 inducible transgenic males activated 1 week
