|
Status |
Public on Jun 30, 2014 |
Title |
Spontaneous IPF, Replicate 1 |
Sample type |
RNA |
|
|
Source name |
Alveolar macrophage isolated from Spontaneous IPF
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: Spontaneous Idiopathic Pulmonary Fibrosis (IPF) tissue: Alveolar Macrophage
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Expression Analysis Technical Manual, 2005, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner.
|
Data processing |
The data were analyzed with Affymetrix apt version 1.12, GeneSpring 11.5.1 . Apt was used to determine present/absent calls and GeneSpring RMA normalization was applied.
|
|
|
Submission date |
Jul 22, 2013 |
Last update date |
Sep 01, 2016 |
Contact name |
Eric Billings |
Organization name |
NHLBI
|
Street address |
10 Center Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20874 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE49072 |
Alveolar Macrophage Gene Expression in Human Pulmonary Fibrosis |
|
Relations |
Reanalyzed by |
GSE86363 |