|
| Status |
Public on Nov 15, 2013 |
| Title |
23PT |
| Sample type |
RNA |
| |
|
| Source name |
Vastus Lateralis muscle biopsy after 27 hours of fasting
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: vastus lateralis muscle
sample type: subjects with Low IGF-I
|
| Treatment protocol |
The biopsies were snapfrozen in liquid nitrogen and stored at -80
|
| Growth protocol |
The skeletal muscle biopsies are obtained after 27 hour fasting and was preceeded by a frequently sampled intravenous glucose tolerance test
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using miRNeasy mini kit (Qiagen), and the miRNA was removed using RNeasy MinElute Cleanup Kit (Qiagen) according to manufacturer´s instructions
|
| Label |
Biotin
|
| Label protocol |
cDNA was generated using Ambion WT Expression Kit (Life Technologies) in accordance with the manufacturerÃs protocol, and was then fragmented and labelled with Affymetrix GeneChipWT Terminal Labeling and Hybridization kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Gene 1.0 ST Arrays (Affymetrix) and scanned at the Genomics Corelab at the Cambridge Biomedical Research Centre.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RNA from Homo Sapiens Vastus Lateralis muscle biopsy after 27 hours of fasting
|
| Data processing |
Raw data were normalized using core gene-level RMA, data analysis and statistical evaluations were performed with Genespring GX12.5
|
| |
|
| Submission date |
Jul 28, 2013 |
| Last update date |
Nov 15, 2013 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE49297 |
Expression data from skeletal muscle biopsies of healthy adults |
|
