|
| Status |
Public on Dec 19, 2013 |
| Title |
hIPod dediff. 3 |
| Sample type |
RNA |
| |
|
| Source name |
hIPod de-differentiated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hIPod
growth condition: Expanded at 33C in RPMI-1640, 10% FBS, 1% Pen/Strep, 1X ITS
tissue: human immortalized podocyte cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of RNA was performed using Qiagen mRNAeasy kit following manufacturer standard protocol
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using Ambion WT Expression Kit (Life Technologies) and cDNA produced.
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized to Affymetrix Human Gene 1.0 ST arrays
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Expanded at 33C in RPMI-1640, 10% FBS, 1% Pen/Strep, 1X ITS
AB061-HuGene-1_0-st-v1-01-1 (C3)
|
| Data processing |
Array data were imported into Partek genomic Suite 6.6 (Partek Incorporated) and quantile normalized using robust multichip averaging (RMA). Following normalization, log2 expression signals from probe sets were summarized in a data table
|
| |
|
| Submission date |
Aug 01, 2013 |
| Last update date |
Dec 20, 2013 |
| Contact name |
Stefano Da Sacco |
| E-mail(s) |
sdasacco@chla.usc.edu
|
| Organization name |
Children's Hospital Los Angeles
|
| Street address |
4650 Sunset Blvd MS#35
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90027 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE49439 |
Differentiation of human amniotic fluid kidney progenitor cells into podocytes and comparison with human conditionally immortalized podocytes |
|
