|
| Status |
Public on Dec 22, 2017 |
| Title |
2-2LD1 |
| Sample type |
RNA |
| |
|
| Source name |
FD transgenic_shoot tips_LD
|
| Organism |
Populus tremula x Populus alba |
| Characteristics |
genotype: transgenic FD overexpressing
tissue: shoot tip
collected after: Long day (LD) photoperiod (16h light/8h dark)
|
| Treatment protocol |
LD = Long day photoperiod (16h light/8h dark) or SD = 3 weeks under short day photoperiod (8h light/16h dark)
|
| Growth protocol |
Plants were grown in growth chambers
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Qiagen RNeasy plant mini kit and Qiacube with modifications
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip 3' IVT expression kit mannual).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 17 hr at 45C on GeneChip Poplar Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000.
|
| Description |
UMCPLin-Poplar46_4-2L1.CEL
2-2 LD replicate 1
|
| Data processing |
The data were analyzed using Genespring software
|
| |
|
| Submission date |
Aug 05, 2013 |
| Last update date |
Dec 22, 2017 |
| Contact name |
Cecile Parmentier-Line |
| Organization name |
University of MD
|
| Street address |
Baltimore avenue
|
| City |
College Park |
| ZIP/Postal code |
20742 |
| Country |
USA |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
|
