|
| Status |
Public on Aug 08, 2013 |
| Title |
Gsvcv_1 |
| Sample type |
RNA |
| |
|
| Source name |
muscle (injection site)
|
| Organism |
Danio rerio |
| Characteristics |
age: adult (+ 6months)
tissue: muscle
treatment: fish injected with the plasmid containing the gene sequence of the gpG of SVCV
|
| Treatment protocol |
Fish (n=4) were intramuscularly injected with 1,5 µg of the plasmid constructs containing the gene sequences of SVCV or VHSV glycoproteins or the empty plasmid. After three days muscle samples were excised from the site of injection and processed from RNA isolation and gene expression analysis with microarrays.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the E.Z.N.A HP Tissue RNA kit (Omega bio-tek) according to intructions provided in the kit. RNA was kept at -80 oC until used.
|
| Label |
Cy3
|
| Label protocol |
Labelling of the samples with Cy3 according to manufacturer instructions (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Each amplified and labelled sample was hybridized at 65 oC for 17 hours.
|
| Scan protocol |
Arrays were scanned and one-channel TIFF (FEATURE EXTRACTION software v. 10.4.0.0.) images were imported into GENESPRING software (GX v. 11.0)
|
| Description |
Gene expression in muscle samples of fish injected with the plasmid containing the gene sequence of the gpG of SVCV (experiment 1)
|
| Data processing |
Data was analysed using GENESPRING software (GX v. 11.0.).Signal intensities for unique probes within a probe set were averaged to obtain an expression value (median) for the probe set (Gene-level analysis). expressed was defined as transcripts with a log2-transformed expression value greater than 1.
|
| |
|
| Submission date |
Aug 07, 2013 |
| Last update date |
Aug 08, 2013 |
| Contact name |
Amparo Estepa Perez |
| Organization name |
University Miguel Hernandez
|
| Department |
Institute of Molecular and Cell Biology
|
| Street address |
Av. Ferrocarril s/n
|
| City |
Elche |
| State/province |
Alicante |
| ZIP/Postal code |
03202 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE49626 |
Study of the differentially-expressed genes induced by the expession of two viral glycoproteins in muscle cells of adult zebrafish |
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