|
| Status |
Public on Aug 09, 2013 |
| Title |
Meg_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Megakaryocytes
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E14.5
tissue: fetal liver
cell type: Megakaryocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Primary megakaryocytes (MEG) were obtained by expansion and differentiation of fetal liver progenitors followed by CD41 immunoselection.
|
| Data processing |
Data analysis was performed using the BioConductor packages ‘affy’library in R. Using the RMA method, hybridization signals were corrected for background, quantile-normalized and log2-transformed.
|
| |
|
| Submission date |
Aug 08, 2013 |
| Last update date |
Aug 09, 2013 |
| Contact name |
Ross Hardison |
| E-mail(s) |
rch8@psu.edu
|
| Organization name |
Pennsylvania State University
|
| Street address |
303 Wartik Lab
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE49664 |
Divergent functions of hematopoietic transcription factors in lineage priming and differentiation during erythro-megakaryopoiesis |
| GSE51337 |
Divergent functions of hematopoietic transcription factors in lineage priming and differentiation during erythro-megakaryopoiesis |
|
