|
| Status |
Public on Sep 22, 2006 |
| Title |
Brain_Wild-type_MiceNoB4394 |
| Sample type |
RNA |
| |
|
| Source name |
whole brain, nicotinic acetylcholine receptor subunit mutant mice and littermate controls
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6J
Gender: male
Age: 8 weeks
Tissue: whole brain
|
| Treatment protocol |
untreated wild-type mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole brains using TRI REAGENT (Sigma-Aldrich, St. Louis, MO) according to the manufacturer?s instructions, and further purified on a Qiagen RNeasy mini kit (Qiagen Sciences, Germantown, MD)
|
| Label |
biotin-labeled nucleotides
|
| Label protocol |
Enzo BioArray High Yield Transcript labeling kit
|
| |
|
| Hybridization protocol |
According to Affymetrix GeneChip expression analysis technical manual
|
| Scan protocol |
scanned with an Affymetrix Gene-Array Scanner according to Affymetrix GeneChip expression analysis technical manual
|
| Description |
whole brain from wild-type littermate of nicotinic acetylcholine receptor subunit mutant mice
|
| Data processing |
MAS5.0, scaled to 150
|
| |
|
| Submission date |
Jul 16, 2006 |
| Last update date |
Sep 22, 2006 |
| Contact name |
Avi Orr-Urtreger |
| E-mail(s) |
aviorr@tasmc.health.gov.il
|
| Phone |
972-3-697-4704
|
| Fax |
972-3-697-4555
|
| Organization name |
Tel Aviv Sourasky Medical Center
|
| Department |
Genetic Institute
|
| Lab |
Research and Development Laboratory
|
| Street address |
6 Weizmann St
|
| City |
Tel Aviv |
| ZIP/Postal code |
64239 |
| Country |
Israel |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5320 |
whole brains from nicotinic acetylcholine receptor subunit mutant mice and littermate controls |
|
