|
| Status |
Public on Aug 02, 2014 |
| Title |
J3-YJ-M |
| Sample type |
RNA |
| |
|
| Source name |
LDM: longissimus dorsi muscle
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Jinhua
gender: female
age: 0.5 years
|
| Treatment protocol |
longissimus dorsi muscle were sampled rapidly from each carcass, and immediately frozen in liquid nitrogen. All tissue samples were stored at − 80 °C until total RNA extraction.
|
| Growth protocol |
pigs aged 0.5 years and 7 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA (10 μg) of the six samples was extracted using TRIzol (Invitrogen) and further purified using an RNeasy column (Qiagen). The integrity of total RNA were also analyzed with the Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
The labeling procedure was carried out using an RNA Fluorescent Linear Amplification Kit (Agilent Technologies). The sample was labeled with Cy-3 dye only.
|
| |
|
| Hybridization protocol |
Fragmentation was carried out by incubation at 60 °C for 30 min in a fragmentation buffer (Agilent Technologies), and the process was stopped by the addition of an equal volume of hybridization buffer (Agilent Technologies).Hybridization was carried out at 60 °C for 17 h in a hybridization oven (Robbins Scientific).
|
| Scan protocol |
The hybridized single channel array was scanned with an Agilent microarray scanner (G2565BA).
|
| Description |
Genome-wide gene expression profile
|
| Data processing |
The TIFF image generated was loaded into Feature Extraction Software (Agilent Technologies) for feature data extraction, and data analysis was performed with MultiExperiment Viewer (MeV). The quantile normalization was performed using GeneSpring 11.5 software.
|
| |
|
| Submission date |
Aug 12, 2013 |
| Last update date |
Aug 03, 2014 |
| Contact name |
long jin |
| E-mail(s) |
jinlong2012benming@gmail.com
|
| Organization name |
Sichuan Agricultural University
|
| Street address |
Ya’an, Xinkang Road #46 Sichuan
|
| City |
Ya'an |
| State/province |
Sichuan |
| ZIP/Postal code |
625014 |
| Country |
China |
| |
|
| Platform ID |
GPL10162 |
| Series (1) |
| GSE49791 |
Genome-wide DNA methylation changes in skeletal muscle between young and middle-aged pigs |
|
