Plants were grown in soil in growth chamber with 16 hours of light for 5 weeks. Immature inflorescence was harvested ~2 hours after dark. Tissue included inflorescence meristem and early stage floral buds (up to Stage 11/12). Total RNA was isolated using TRIzol reagent.
Data processing
To determine the normalized abundance value for each signature in the library, we separately merged 2-step runs and 3-step runs to create a raw abundance count for each stepper. The raw abundance count for each stepper was calculated as the average from all 2-step or from all 3-step runs for a given signature. The average total number of signatures sequenced in all 2-step or for all 3-step runs was also calculated within the library. The final stage to merge the data within the steppers was to calculate the normalized abundance for each stepper. The normalized abundance is the raw abundance count divided by the average total number of signatures for both of the steppers in the library, multiplied by 250,000 to obtain a
