|
| Status |
Public on Aug 21, 2013 |
| Title |
Broiler2_39152428 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Broiler2
|
| Organism |
Gallus gallus |
| Characteristics |
gender: male
line: Broiler2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated with the oPuregene blood kit of the Qiagen Qiamap DNA blood kit.
|
| Label |
Cy5
|
| Label protocol |
for each DNA sample 2.5 μg ws used for the labeling experiments using the Agilent Genomic DNA Labeling kit PLUS. Afterwards the concentration and fluorescent dye incorporation of the labeled fragments were measured using the nanodrop. For each array equal amounts of fluorescent DNA is used for hybridization
|
| |
|
| Channel 2 |
| Source name |
UCD001 reference
|
| Organism |
Gallus gallus |
| Characteristics |
line: UCD001
gender: male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated with the oPuregene blood kit of the Qiagen Qiamap DNA blood kit.
|
| Label |
Cy3
|
| Label protocol |
for each DNA sample 2.5 μg ws used for the labeling experiments using the Agilent Genomic DNA Labeling kit PLUS. Afterwards the concentration and fluorescent dye incorporation of the labeled fragments were measured using the nanodrop. For each array equal amounts of fluorescent DNA is used for hybridization
|
| |
|
| |
| Hybridization protocol |
Agilent CHG protocols (for FFPE samples) version 1.02007
|
| Scan protocol |
Agilent CHG protocols (for FFPE samples) version 1.02007 were stricktly followed for scanning . The arrays were scanned with 100% PMT laser power.
|
| Description |
0003_
|
| Data processing |
the feature extraction software version 9.5, protocvol CGH-v4_95_feb07 from Agilent was used to generate te feature extraction data.
|
| |
|
| Submission date |
Aug 20, 2013 |
| Last update date |
Aug 21, 2013 |
| Contact name |
richard p.m.a. crooijmans |
| E-mail(s) |
richard.crooijmans@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Animal Breeding and Genomics Centre
|
| Street address |
de Elst 1
|
| City |
Wageningen |
| ZIP/Postal code |
6708 WD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL15501 |
| Series (1) |
| GSE47623 |
Large scale variation in copy number in chicken breeds |
|
