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Sample GSM1218141 Query DataSets for GSM1218141
Status Public on Feb 01, 2014
Title Postnatal 3 weeks mutant heart biological rep2
Sample type RNA
 
Source name postnatal 3 weeks pitx2 cko heart
Organism Mus musculus
Characteristics age: 3 wk
tissue: whole heart
genotype: MCKcre Pitx2 Flox/Flox
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by using Trizol (GIBCOyBRL). RNA pellets were resuspended at 1 ug RNA/ul diethyl pyrocarbonate-treated water.
Label biontin
Label protocol 8 ug of total RNA was used in a reverse transcription reaction (SuperScript II; Life Technologies, Rockville, MD) to generate first-strand cDNA. After second-strand synthesis, double-strand cDNA was used in an in vitro transcription (IVT) reaction to generate biotinylated cRNA.
 
Hybridization protocol After purification and fragmentation, 15ug of cRNA was used in a 300 ul hybridization mixture containing spiked IVT controls. Approximately 200 ul of mixture was hybridized on chips for 16 h at 45°C. Standard posthybridization wash and double-stain protocols used an Affymetrix GeneChip Fluidics Station 400.
Scan protocol Arrays were scanned by GeneChip Scanner 3000
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 28, 2013
Last update date Feb 01, 2014
Contact name Min Zhang
E-mail(s) minzhang5099@gmail.com
Organization name Baylor College of Medicine
Department Department of Molecular Physiology
Lab James F Martin
Street address One Baylor Plaza Room 504E
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL1261
Series (2)
GSE50399 Gene expression data of postnatal Pitx2 deficient heart
GSE50401 Pitx2 in the postnatal heart

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1490.1
1415671_at 2023.3
1415672_at 4229.1
1415673_at 500.9
1415674_a_at 1192.6
1415675_at 490.7
1415676_a_at 4644.4
1415677_at 793
1415678_at 4232.3
1415679_at 3149.5
1415680_at 1175.9
1415681_at 2206.7
1415682_at 683.8
1415683_at 3884.9
1415684_at 641
1415685_at 2770.9
1415686_at 1960.8
1415687_a_at 14333
1415688_at 5995.9
1415689_s_at 920.6

Total number of rows: 45101

Table truncated, full table size 739 Kbytes.




Supplementary file Size Download File type/resource
GSM1218141_3wks_cko2.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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