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Sample GSM1218144 Query DataSets for GSM1218144
Status Public on Feb 01, 2014
Title Postnatal 6 weeks control heart biological rep2
Sample type RNA
 
Source name postnatal 6 weeks control heart
Organism Mus musculus
Characteristics age: 6 wk
tissue: whole heart
genotype: Pitx2 Flox/Flox
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by using Trizol (GIBCOyBRL). RNA pellets were resuspended at 1 ug RNA/ul diethyl pyrocarbonate-treated water.
Label biontin
Label protocol 8 ug of total RNA was used in a reverse transcription reaction (SuperScript II; Life Technologies, Rockville, MD) to generate first-strand cDNA. After second-strand synthesis, double-strand cDNA was used in an in vitro transcription (IVT) reaction to generate biotinylated cRNA.
 
Hybridization protocol After purification and fragmentation, 15ug of cRNA was used in a 300 ul hybridization mixture containing spiked IVT controls. Approximately 200 ul of mixture was hybridized on chips for 16 h at 45°C. Standard posthybridization wash and double-stain protocols used an Affymetrix GeneChip Fluidics Station 400.
Scan protocol Arrays were scanned by GeneChip Scanner 3000
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 28, 2013
Last update date Feb 01, 2014
Contact name Min Zhang
E-mail(s) minzhang5099@gmail.com
Organization name Baylor College of Medicine
Department Department of Molecular Physiology
Lab James F Martin
Street address One Baylor Plaza Room 504E
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL1261
Series (2)
GSE50399 Gene expression data of postnatal Pitx2 deficient heart
GSE50401 Pitx2 in the postnatal heart

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1571.3
1415671_at 2360.7
1415672_at 3989.8
1415673_at 402.6
1415674_a_at 1340.2
1415675_at 455.6
1415676_a_at 5926.6
1415677_at 851.8
1415678_at 4469.7
1415679_at 3200.9
1415680_at 1184.8
1415681_at 2732.1
1415682_at 795
1415683_at 4176.7
1415684_at 790.2
1415685_at 2375.5
1415686_at 1521.4
1415687_a_at 20691.6
1415688_at 5386
1415689_s_at 714.6

Total number of rows: 45101

Table truncated, full table size 738 Kbytes.




Supplementary file Size Download File type/resource
GSM1218144_6wks_ctrl2.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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