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Sample GSM1220782 Query DataSets for GSM1220782
Status Public on Nov 10, 2013
Title GFP replicate 2
Sample type RNA
 
Source name Hepatoblasts expressing GFP 8hrs
Organism Mus musculus
Characteristics cell type: Embryonic hepatoblasts
Growth protocol Hepatoblasts were seeded onto gelatin coated plates. Medium was DMEM (sigma D0819), plus 10% FBS, 1% penicillin/streptomycin, HGF 10ng/ml, EGF 20 ng/ml, dexamethasone 1uM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Rneasy columns (QIAgen 74104)
Label biotin
Label protocol RNA was reverse transcribed using an Ovation Pico WTA kit (NuGen, 3300-12) to produce an amplified cDNA. Second strand synthesis was achieved using a WT-Ovation Exon module version 1.0 kit (NuGen, 2000-12), after which the cDNA was fragmented and labelled using Encore Biotin Module reagents (NuGen, 4200-12).
 
Hybridization protocol The products were hybridized to GeneChip Mouse Gene Array 1.0 ST (Affymetrix) microarray chips. Hybridized arrays were washed using a GeneChip Fluidics Station 450 (Affymetrix).
Scan protocol Scanned using a GeneChip Scanner 30007G (Affymetrix).
Data processing The data were analyzed with RMA implemented in the software apt-probeset-summarize with the default settings.
 
Submission date Sep 03, 2013
Last update date Nov 10, 2013
Contact name steve lyons
E-mail(s) slyons@picr.man.ac.uk
Phone 0161 446 3028
Organization name CRUK Manchester
Department Cell Regulation
Street address Wilmslow Rd
City Manchester
ZIP/Postal code M20 4BX
Country United Kingdom
 
Platform ID GPL10740
Series (1)
GSE50530 Identification of ATF2 target genes

Data table header descriptions
ID_REF
VALUE log2 of the signal intensity, defined in apt-probeset-summarize

Data table
ID_REF VALUE
10338001 11.58265
10338002 4.64534
10338003 9.66181
10338004 6.40468
10338005 1.61547
10338006 1.75743
10338007 1.97011
10338008 2.61837
10338009 5.44803
10338010 1.70031
10338011 4.16294
10338012 1.67636
10338013 1.49174
10338014 1.59853
10338015 1.50878
10338016 5.46635
10338017 12.60519
10338018 4.96973
10338019 3.69776
10338020 5.89558

Total number of rows: 241576

Table truncated, full table size 3999 Kbytes.




Supplementary file Size Download File type/resource
GSM1220782.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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