|
Status |
Public on Nov 10, 2013 |
Title |
GFP replicate 3 |
Sample type |
RNA |
|
|
Source name |
Hepatoblasts expressing GFP 8hrs
|
Organism |
Mus musculus |
Characteristics |
cell type: Embryonic hepatoblasts
|
Growth protocol |
Hepatoblasts were seeded onto gelatin coated plates. Medium was DMEM (sigma D0819), plus 10% FBS, 1% penicillin/streptomycin, HGF 10ng/ml, EGF 20 ng/ml, dexamethasone 1uM.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Rneasy columns (QIAgen 74104)
|
Label |
biotin
|
Label protocol |
RNA was reverse transcribed using an Ovation Pico WTA kit (NuGen, 3300-12) to produce an amplified cDNA. Second strand synthesis was achieved using a WT-Ovation Exon module version 1.0 kit (NuGen, 2000-12), after which the cDNA was fragmented and labelled using Encore Biotin Module reagents (NuGen, 4200-12).
|
|
|
Hybridization protocol |
The products were hybridized to GeneChip Mouse Gene Array 1.0 ST (Affymetrix) microarray chips. Hybridized arrays were washed using a GeneChip Fluidics Station 450 (Affymetrix).
|
Scan protocol |
Scanned using a GeneChip Scanner 30007G (Affymetrix).
|
Data processing |
The data were analyzed with RMA implemented in the software apt-probeset-summarize with the default settings.
|
|
|
Submission date |
Sep 03, 2013 |
Last update date |
Nov 10, 2013 |
Contact name |
steve lyons |
E-mail(s) |
slyons@picr.man.ac.uk
|
Phone |
0161 446 3028
|
Organization name |
CRUK Manchester
|
Department |
Cell Regulation
|
Street address |
Wilmslow Rd
|
City |
Manchester |
ZIP/Postal code |
M20 4BX |
Country |
United Kingdom |
|
|
Platform ID |
GPL10740 |
Series (1) |
GSE50530 |
Identification of ATF2 target genes |
|