|
| Status |
Public on Sep 05, 2013 |
| Title |
Keratinocytes untreated 1h |
| Sample type |
RNA |
| |
|
| Source name |
Keratinocytes untreated 1h
|
| Organism |
Homo sapiens |
| Characteristics |
timpe point: 1h
cell type: Epidermal keratinocytes
treatment: Untreated control
|
| Treatment protocol |
~70% confluent keratinocytes treated with 5 uM JNK inhibitor SP600125, 15 uM p38 inhibitor SB203580, or 50 um ERK inhibitor PD98059.
|
| Growth protocol |
Standard e.g. as in Banno et al., J Biol Chem 279(31): 32633-32642.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard e.g. as in Banno et al., J Biol Chem 279(31): 32633-32642.
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Ctrl-1hr-exp I
|
| Data processing |
MAS5 GCOS, RMAExpress summarized using PLM and log2 transformed
|
| |
|
| Submission date |
Sep 04, 2013 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Miroslav Blumenberg |
| E-mail(s) |
miroslav.blumenberg@nyumc.org
|
| Phone |
212 263-5924
|
| Organization name |
NYU School of Medicine
|
| Department |
Dermatology
|
| Lab |
Blumenberg
|
| Street address |
455 First Ave #874
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE50591 |
Transcriptional Profiling Defines the Roles of ERK and p38 Kinases in Epidermal Keratinocytes |
|
