|
| Status |
Public on Sep 06, 2013 |
| Title |
Gestational nicotine exposure, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Gestationally exposed to nicotine, killed at PND35-42
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Striatum
strain: Lister hooded
|
| Treatment protocol |
Striatal samples were snap frozen on dry ice and stored at -80 degrees until RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen AllPrep RNA/DNA min ikits
|
| Label |
biotin
|
| Label protocol |
10 ug of cRNA was reverse transcribed and 5.5ug of ssDNA was fragmented and biotin labelled using the Ambion WT expression kit (manufacturers guidelines).
|
| |
|
| Hybridization protocol |
cDNA was hybridised to the Affy rat GeneChip 1.0 ST array for 16 hours at 45 degrees. Washing and staining was performed on the Affymetrix fluidics platform.
|
| Scan protocol |
Stained arrays were scanned using the Affymetrix GeneChip scanner 3000.
|
| Description |
Gene expression data from nicotine exposed striatum
|
| Data processing |
Probe set summaries were processed using the RMA algorithm using the Affymetrix gene expression console v1.1 software. Gene-default parameters were used (14/01/2010)
|
| |
|
| Submission date |
Sep 05, 2013 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Nicholas Edward Ilott |
| E-mail(s) |
nicholas.ilott@dpag.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE50607 |
Gestational food-restriction but not nicotine exposure regulates gene expression in the striatum of adolescent rats |
|
