|
| Status |
Public on Sep 05, 2013 |
| Title |
mAb 75G12 |
| Sample type |
protein |
| |
|
| Source name |
VH4-34 Switched memory B cell generated monoclonal antibody
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: SLE
cell type: VH4-34 Switched memory B cell
purified monoclonal antibody: mAb 75G12
concentration: 10ug/ml
|
| Growth protocol |
Single VH4-34 B cells sorted from IgD-CD27+ memory cells of SLE patient
|
| Extracted molecule |
protein |
| Extraction protocol |
Monoclonal antibody was obtained by single cell RT-PCR followed by cloning and expressing into 293 cell system. Supernatant was concentrated and quantified by IgG ELISA. Crude was purified by dialysis and 10ug/ml purified monoclonla antibody was used on the autoantigen array
|
| Label |
Cy3-conjugated anti-human IgG antibody
|
| Label protocol |
autoantibodies binding to the antigens on the array detected with laser wavelengths 532nm (cy3 labeled anti-IgG)
|
| |
|
| Hybridization protocol |
Autoantigen array protocol (microarray core, University of Texas Southwestern Medical Center, Dallas, TX
|
| Scan protocol |
Genepix Pro 6.0 software is used to analyze the image and generate the .GPR file (GenePix Results Format)
|
| Description |
Purified 10ug/ml of monoclonal antibody is incubated with autoantigen array
|
| Data processing |
All the data is normalized by the positive control human IgG or human IgM after subtracted by the value of PBS (the negative control).
|
| |
|
| Submission date |
Sep 05, 2013 |
| Last update date |
Sep 05, 2013 |
| Contact name |
yun lian |
| E-mail(s) |
yun.lian@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL17684 |
| Series (1) |
| GSE50609 |
Molecular basis of 9G4 B cell autoreactivity in human SLE |
|
