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Sample GSM1224646 Query DataSets for GSM1224646
Status Public on Sep 05, 2013
Title mAb 75G12
Sample type protein
 
Source name VH4-34 Switched memory B cell generated monoclonal antibody
Organism Homo sapiens
Characteristics disease state: SLE
cell type: VH4-34 Switched memory B cell
purified monoclonal antibody: mAb 75G12
concentration: 10ug/ml
Growth protocol Single VH4-34 B cells sorted from IgD-CD27+ memory cells of SLE patient
Extracted molecule protein
Extraction protocol Monoclonal antibody was obtained by single cell RT-PCR followed by cloning and expressing into 293 cell system. Supernatant was concentrated and quantified by IgG ELISA. Crude was purified by dialysis and 10ug/ml purified monoclonla antibody was used on the autoantigen array
Label Cy3-conjugated anti-human IgG antibody
Label protocol autoantibodies binding to the antigens on the array detected with laser wavelengths 532nm (cy3 labeled anti-IgG)
 
Hybridization protocol Autoantigen array protocol (microarray core, University of Texas Southwestern Medical Center, Dallas, TX
Scan protocol Genepix Pro 6.0 software is used to analyze the image and generate the .GPR file (GenePix Results Format)
Description Purified 10ug/ml of monoclonal antibody is incubated with autoantigen array
Data processing All the data is normalized by the positive control human IgG or human IgM after subtracted by the value of PBS (the negative control).
 
Submission date Sep 05, 2013
Last update date Sep 05, 2013
Contact name yun lian
E-mail(s) yun.lian@utsouthwestern.edu
Organization name UT Southwestern Medical Center
Street address 5323 Harry Hines Blvd.
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platform ID GPL17684
Series (1)
GSE50609 Molecular basis of 9G4 B cell autoreactivity in human SLE

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
Aggencan 0.086883408
alpha-actinine 2.589375209
Amyloid 0.045837646
Annexin V 5.282879082
B2-glycoprotein I 32.78807151
B2-microglobulin 28.75409295
BPI 9.727214516
C1Q 3.467990915
Cardolipin 5.934377274
CENP-A 19.28949339
CENP-B 59.4708286
Chondroitin sulfate C 0.004950199
Chromatin 8.432514647
Collagen I 9.920000001
Collagen II 35.21885285
Collagen V 13.90737406
Collagen VI 0.000282934
Cytochrome C 10.40357476
DGPS 4.493213287
ds RNA 96.15350709

Total number of rows: 85

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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