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Status |
Public on Sep 05, 2013 |
Title |
mAb 75G12 |
Sample type |
protein |
|
|
Source name |
VH4-34 Switched memory B cell generated monoclonal antibody
|
Organism |
Homo sapiens |
Characteristics |
disease state: SLE cell type: VH4-34 Switched memory B cell purified monoclonal antibody: mAb 75G12 concentration: 10ug/ml
|
Growth protocol |
Single VH4-34 B cells sorted from IgD-CD27+ memory cells of SLE patient
|
Extracted molecule |
protein |
Extraction protocol |
Monoclonal antibody was obtained by single cell RT-PCR followed by cloning and expressing into 293 cell system. Supernatant was concentrated and quantified by IgG ELISA. Crude was purified by dialysis and 10ug/ml purified monoclonla antibody was used on the autoantigen array
|
Label |
Cy3-conjugated anti-human IgG antibody
|
Label protocol |
autoantibodies binding to the antigens on the array detected with laser wavelengths 532nm (cy3 labeled anti-IgG)
|
|
|
Hybridization protocol |
Autoantigen array protocol (microarray core, University of Texas Southwestern Medical Center, Dallas, TX
|
Scan protocol |
Genepix Pro 6.0 software is used to analyze the image and generate the .GPR file (GenePix Results Format)
|
Description |
Purified 10ug/ml of monoclonal antibody is incubated with autoantigen array
|
Data processing |
All the data is normalized by the positive control human IgG or human IgM after subtracted by the value of PBS (the negative control).
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|
|
Submission date |
Sep 05, 2013 |
Last update date |
Sep 05, 2013 |
Contact name |
yun lian |
E-mail(s) |
yun.lian@utsouthwestern.edu
|
Organization name |
UT Southwestern Medical Center
|
Street address |
5323 Harry Hines Blvd.
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL17684 |
Series (1) |
GSE50609 |
Molecular basis of 9G4 B cell autoreactivity in human SLE |
|