|
| Status |
Public on Sep 20, 2016 |
| Title |
Palapattu_016_UDP_MDV4 |
| Sample type |
RNA |
| |
|
| Source name |
C4-2 cells after 96 hours of UDP-GlcNAc + MDV3100 treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: C4-2
cell type: Prostate cancer
|
| Treatment protocol |
100,000 cells/well in 24 well plate were grown and 15 mM UDP-N-acetylglucosamine or 10uM MDV 3100 alone or in combination were added to the cells for 96 hours
|
| Growth protocol |
C42 cells were grown in grown in T-media with 10 % Fetal Bovine with and 1% PenStrip
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Extracted RNA samples were send for microarray using Human Affymetrix Gene ST 2.1
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Affymetrix Gene ST 2.1 . Chips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
Gene expression analysis with values for each gene being calculated using robust multi-array average (RMA).
|
| |
|
| Submission date |
Sep 06, 2013 |
| Last update date |
Sep 20, 2016 |
| Contact name |
Akash Kumar Kaushik |
| E-mail(s) |
akkaushi@bcm.edu
|
| Phone |
9362050781
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
Sreekumar Lab 120D
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE50661 |
Pathway based integrative analysis reveals a key role for the hexosamine biosynthetic pathway in prostate cancer progression with therapeutic implications |
|
