|
| Status |
Public on Jan 01, 2017 |
| Title |
CD3 lymphocytes from MD44 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
CD3 lymphocytes isolated from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: F
who: RCMD
iPSs: low
tissue: peripheral blood
cell type: CD3 lymphocytes
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole bone marrow, whole peripheral blood or CD3 lymphocytes isolated from peripheral blood at diagnosis. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
Protocol Version 6.2.1, Feb 2010, Agilent. Same gender reference/control DNA was purchased from Kreatech as pool.
|
| |
|
| Channel 2 |
| Source name |
control DNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: gender-matched, pooled reference/control DNA (Kreatech)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole bone marrow, whole peripheral blood or CD3 lymphocytes isolated from peripheral blood at diagnosis. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Protocol Version 6.2.1, Feb 2010, Agilent. Same gender reference/control DNA was purchased from Kreatech as pool.
|
| |
|
| |
| Hybridization protocol |
Protocol Version 6.2.1, Feb 2010, Agilent.
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version 10.7.1.1).
|
| Description |
Sample name: MD44T
|
| Data processing |
Agilent Feature Extraction Software (version 10.7.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 16, 2013 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Brigitte Royer-Pokora |
| E-mail(s) |
royer@uni-duesseldorf.de
|
| Organization name |
Institute of Human Genetics and Anthropology, Heinrich-Heine-University Duesseldorf
|
| Street address |
Universitätsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE50897 |
Bioinformatic evaluation and comparison of parallel aSNP and aCGH analyses of myelodysplastic syndromes patients with normal karyotype |
|
