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Sample GSM1234842 Query DataSets for GSM1234842
Status Public on Feb 15, 2016
Title Heart, Infarcted, rep2
Sample type RNA
 
Source name Heart, Infarcted
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: heart
age: 12 week-old
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol (Invitrogen). RNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 BioanalyzerRNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 Bioanalyzer.
Label biotin
Label protocol 200ng of total RNA were used to perform target preparation using the WT Expression Kit (Life Technologies). 5.5 μg of each fragmented cDNA was end-labeled with biotin
 
Hybridization protocol Arrays (Affymetrix, Santa Clara , CA, USA) were hybridized with 2.7μg of biotinylated target, at 45°C for 17 hours washed and stained according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_0007).
Scan protocol Arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix)
Description 12 week old mice, border zone of infarct, 2 weeks after surgery
Data processing Background subtraction, quantile normalization and probeset summarization was performed using the Affymetrix Power Tools apt-probeset-summarize command with the -rma option
 
Submission date Sep 19, 2013
Last update date Feb 16, 2016
Contact name Mark Ibberson
Organization name SIB Swiss Institute of Bioinformatics
Department Vital-IT
Street address Genopode building
City Lausanne
ZIP/Postal code CH-1015
Country Switzerland
 
Platform ID GPL10740
Series (2)
GSE51012 Comparative transcriptome profiling of the injured zebrafish and mouse hearts identifies miRNA-dependent repair pathways
GSE51014 Comparative transcriptome profiling of the injured zebrafish and mouse hearts identifies miRNA-dependent repair pathways

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
10338001 12.04288
10338002 6.40979
10338003 10.29149
10338004 9.25038
10338005 2.4715
10338006 2.68318
10338007 2.94932
10338008 3.5144
10338009 8.37176
10338010 2.52509
10338011 5.80747
10338012 2.58293
10338013 2.37814
10338014 2.42897
10338015 2.343
10338016 7.68099
10338017 13.14161
10338018 6.90675
10338019 5.08644
10338020 8.2299

Total number of rows: 241576

Table truncated, full table size 4009 Kbytes.




Supplementary file Size Download File type/resource
GSM1234842_13R_hf5.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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