|
| Status |
Public on Feb 11, 2014 |
| Title |
hiPSC-EpSC-2 |
| Sample type |
RNA |
| |
|
| Source name |
human iPSC-derived epithelial stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC-derived epithelial stem cells
tissue origin: normal skin
|
| Growth protocol |
Human iPSCs and EpSCs were cultured before they reached 70% confluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed again with HBSS and lysed directly in TRI Reagent® RNA Isolation Reagent (Sigma) and stored at -20°C.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
iPS-keratinocyte-2
iPS.K.2
|
| Data processing |
The data were normalised using quantile normalisation with lumi in R.
|
| |
|
| Submission date |
Sep 19, 2013 |
| Last update date |
Feb 11, 2014 |
| Contact name |
Xiaowei Xu |
| E-mail(s) |
georgexu08@gmail.com
|
| Phone |
2156626503
|
| Organization name |
University of Pennsylvania
|
| Street address |
3400 Spruce st
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE51028 |
Gene expression profiles among hiPSCs, hiPSC-EpSCs and hEpSCs |
|
