|
| Status |
Public on Jun 30, 2015 |
| Title |
embryonic ovary Wnt4 KO female_E12.5-2 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic ovary at E12.5 biological rep2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embryonic gonad
genotype: Wnt4 knock-out
age: Embryonic day 12.5
|
| Growth protocol |
Embryonic gonad were dissected and frozen immediately until PCR genotyping was carried out.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Set 430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GenChip Scanner 3000.
|
| Description |
Gene expression data from embryonic gonad
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 23, 2013 |
| Last update date |
Jun 30, 2015 |
| Contact name |
Florence Naillat |
| E-mail(s) |
florence.naillat@oulu.fi
|
| Organization name |
Oulu University
|
| Street address |
Aapistie 5A
|
| City |
Oulu |
| ZIP/Postal code |
90220 |
| Country |
Finland |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE51089 |
Expression data from E12.5 and E14.5 mouse embryonic gonad of wild type (WT) and Wnt-4 knock-out (KO) mice. [Mouse430_2] |
| GSE52730 |
Expression data from E12.5 and E14.5 mouse embryonic gonad of wild type (WT) and Wnt-4 knock-out (KO) mice. |
|
