NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1239350 Query DataSets for GSM1239350
Status Public on Jun 04, 2014
Title N487T4
Sample type RNA
 
Source name Cord blood_#487_CD4 Tcell_CCR4
Organism Homo sapiens
Characteristics donor id: #487
gender: male
tissue: cord blood
cell type: CD4 T cells
cell subset: CCR4
Treatment protocol Activaiton with antiCD3+antiCD28
Growth protocol Culture for 24h in RPMI with 10%FCS
Extracted molecule total RNA
Extraction protocol Cell samples were lysed using SuperAmp Lysis Buffer following manufacturer’s instructions. SuperAmplification was performed according to Miltenyi Biotec’s undisclosed protocol. Briefly, the amplification is based on a global PCR protocol of mRNA-derived cDNA. mRNA was isolated via magnetic bead technology.
Label Cy3
Label protocol 250 ng of each of the amplified cDNAs were used as template for Cy3 labeling which was performed according to Miltenyi Biotec’s undisclosed protocol.
 
Hybridization protocol Cy3 labeled cDNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Human Genome Oligo Microarrays (8x60K) using Agilent’s recommended hybridization chamber and oven. Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
Description 39
Data processing The Agilent Feature Extraction Software (FES 10.7.3.1) was used to read out and process the microarray image files.
 
Submission date Sep 24, 2013
Last update date Jun 04, 2014
Contact name Richard Lo-Man
E-mail(s) richard.lo-man@pasteur.fr
Organization name Institut Pasteur
Lab Neonatal Immunity group
Street address 25-28 Rue du Docteur Roux
City Paris
ZIP/Postal code 75015
Country France
 
Platform ID GPL16699
Series (1)
GSE51133 Analysis of cord blood CD4T cells

Data table header descriptions
ID_REF
VALUE median normalized signal intensities

Data table
ID_REF VALUE
4 2.916677
5 0.835216
6 0.372977
7 17.63059
8 0.37577
9 5.131558
10 0.378019
11 0.37894
12 0.379675
13 672.346403
14 7.685479
15 0.381306
16 0.543683
17 104.256729
18 8.764491
19 2.692294
20 0.381589
21 1.020512
22 0.380959
23 0.380379

Total number of rows: 58717

Table truncated, full table size 862 Kbytes.




Supplementary file Size Download File type/resource
GSM1239350_253949412159_S01_GE1_107_Sep09_1_1.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.